Recombinant Human Zinc Transporter Zip6 (SLC39A6) Protein (His), Active

Name: Recombinant Human Zinc Transporter Zip6 (SLC39A6) Protein (His), Active
Brand: Beta LifeScience
SKU: BLC-05737P
Price: 349.0 USD
Availability: InStock

Greater than 95% as determined by SDS-PAGE.

Activity Measured by its binding ability in a functional ELISA. Immobilized Human SLC39A6 at 1 μg/ml can bind Anti-SLC39A6 recombinant antibody , the EC 50 is 0.6873-0.9010 ng/mL.

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Size

100ug

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Product Overview

Description	Recombinant Human Zinc Transporter Zip6 (SLC39A6) Protein (His), Active is produced by our Baculovirus expression system. This is a protein fragment.
Purity	Greater than 95% as determined by SDS-PAGE.
Endotoxin	Less than 1.0 EU/ug as determined by LAL method.
Activity	Measured by its binding ability in a functional ELISA. Immobilized Human SLC39A6 at 1 μg/mL can bind Anti- SLC39A6 recombinant antibody , the EC50 is 0.6873-0.9010 ng/mL.
Uniprotkb	Q13433
Target Symbol	SLC39A6
Synonyms	(Estrogen-regulated protein LIV-1)(Solute carrier family 39 member 6)(Zrt- and Irt-like protein 6)(ZIP-6)
Species	Homo sapiens (Human)
Expression System	Baculovirus
Tag	C-6His
Target Protein Sequence	FPQTTEKISPNWESGINVDLAISTRQYHLQQLFYRYGENNSLSVEGFRKLLQNIGIDKIKRIHIHHDHDHHSDHEHHSDHERHSDHEHHSEHEHHSDHDHHSHHNHAASGKNKRKALCPDHDSDSSGKDPRNSQGKGAHRPEHASGRRNVKDSVSASEVTSTVYNTVSEGTHFLETIETPRPGKLFPKDVSSSTPPSVTSKSRVSRLAGRKTNESVSEPRKGFMYSRNTNENPQECFNASKLLTSHGMGIQVPLNATEFNYLCPAIINQIDARSCLIHTSEKKAEIPPKTYSLQIAW
Expression Range	29-325aa
Protein Length	Partial
Mol. Weight	35.0 kDa
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	May act as a zinc-influx transporter.
Subcellular Location	Cell membrane; Multi-pass membrane protein.
Protein Families	ZIP transporter (TC 2.A.5) family
Database References	HGNC: 18607 OMIM: 608731 KEGG: hsa:25800 STRING: 9606.ENSP00000269187 UniGene: Hs.79136
Tissue Specificity	Highly expressed in the breast, prostate, placenta, kidney, pituitary and corpus callosum. Weakly expressed in heart and intestine. Also highly expressed in cells derived from an adenocarcinoma of the cervix and lung carcinoma.

Gene Functions References

ZIP6 deficiency disturbs intracellular Zn2+ homeostasis, leading to increased cell survival in hypoxia and reduced E-cadherin expression, indicating that decreased ZIP6 expression is strongly associated with resistance to hypoxia. PMID: 28833062
SLC39A6 promotes aggressiveness of esophageal carcinoma cells by increasing intracellular levels of zinc, activating phosphatidylinositol 3-kinase signaling, and up-regulating genes that regulate metastasis. PMID: 28209530
Upregulation of SLC39A6 is associated with hepatocellular carcinoma. PMID: 26684241
SLC39A6 may have a tumor promoting role in esophageal carcinoma PMID: 26444413
Knock-down of ZIP6 but not ZIP7 in MIN6 beta cells impaired the protective effects of GLP-1 on fatty acid-induced cell apoptosis, possibly via reduced activation of the p-ERK pathway PMID: 25969539
drug resistance of ovarian cancer cells to trichostatin A may be related to expression of the LIV1 gene PMID: 25420545
Zinc and its transporters, ZIP6 and ZIP10, are required for the breast cancer cells motility stimulated with high glucose level, such as in diabetes. PMID: 24587242
a causative role for ZIP6 in cell motility and migration PMID: 23919497
Down-regulated LIV-1 cells showed significant inhibition of proliferation in vitro and reduction of tumor growth in vivo. Furthermore, E-cadherin expression increased in LIV-1 siRNA expressing Hep-G2. PMID: 23437163
SLC39A6 has an important role in the prognosis of esophageal squamous-cell carcinoma and may be a potential therapeutic target. PMID: 23644492
Some studies correlate LIV-1 expression with a more aggressive cancer phenotype and increased likelihood for metastasis to lymph nodes. In contrast, other evidence suggests this transporter is associated with a more favorable prognosis. [review] PMID: 22852056
The results of this study showed evidence for a positive correlation between LIV1 and ZnT6 insuperior temporal, occipital, and frontal gyri in patient with alzheimer disease. PMID: 22349685
LIV-1 is involved in prostate cancer progression as an intracellular target of growth factor receptor signaling which promoted EMT and cancer metastasis PMID: 22110740
LIV-1 mRNA upregulation is associated with the progression of cervical cancer but not with the development of endometrial carcinoma. PMID: 17959546
The present study identifies LIV1 as a critical mediator responsible for HDACi-induced apoptosis. The effect of LIV1 is, at least in part, mediated by affecting intracellular zinc homeostasis. PMID: 19887557
Zip6 over-expression is not an underlying mechanism initiating breast cancer, but in fact may play a "tumor-constraining" role. PMID: 19852955
Structure-function analysis of LIV-1. PMID: 12839489
data suggest that LIV-1 protein is a promising candidate for a novel marker for breast cancer patients with better outcome PMID: 15986450
regulation of LIV-1 protein in human breast cancer xenografts PMID: 17786585
Hence, our data provide the first evidence that LIV-1 mRNA is overexpressed in cervical cancer in situ and is involved in invasion of cervical cancer cells through targeting MAPK-mediated Snail and Slug expression. PMID: 17825787
LIV-1 may be a regulator of E-cadherin PMID: 18330719
Single nucleotide polymorphism in SLC39A6 gene is associated with acute lymphoblastic leukemia. PMID: 19066393
LIV-1 enhances the aggressive phenotype through the induction of epithelial to mesenchymal transition in human pancreatic carcinoma cells. PMID: 19724917
LIV-1 co-clusters with estrogen receptor alpha in microarray analysis of breast cancer biopsies. PMID: 11911440
Review of LIV-1 and other LZT proteins. PMID: 12659941
LIV-1, a member of the ZIP family of zinc transporters, is an integral plasma membrane protein that transports zinc into cells. PMID: 12839489
LIV-1 is coregulated with estrogen receptor in some breast cancers. PMID: 12960427

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.