Recombinant Mouse AFP Protein (C-10His)

Name: Recombinant Mouse AFP Protein (C-10His)
Brand: Beta LifeScience
SKU: BL-1982NP
Availability: InStock

BL-1982NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Other recombinant proteins, Recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Description	Recombinant Mouse Alpha-fetoprotein is produced by our Mammalian expression system and the target gene encoding Lys19-Val605 is expressed with a 10His tag at the C-terminus.
Accession	P02772
Synonym	Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; AFP; Afp
Gene Background	Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitaminD (Gc) protein, and alpha-albumin. AFP is a major plasma protein produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and bilirubin and is found in monomeric, dimeric and trimeric forms. AFP is one of the several embryo-specific proteins and is adominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver (1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignnt growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).
Molecular Mass	67 KDa
Apmol Mass	70-75 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Binds estrogens, fatty acids and metals.
Subcellular Location	Secreted.
Protein Families	ALB/AFP/VDB family
Database References	KEGG: mmu:11576 STRING: 10090.ENSMUSP00000041006 UniGene: Mm.358570
Tissue Specificity	Plasma.

Gene Functions References

endogenous AFP expression in the perinatal liver is reduced in the absence of beta-catenin, suggesting that beta-catenin regulates AFP during liver development. PMID: 22544812
Findings indicate that AFP expressed under experimental cryptorchid conditions plays a role as a regulatory factor in spermatogenesis and in hepatic generation. PMID: 21573244
AFP mRNA and VEGF-C mRNA in peripheral blood were significantly correlated with recurrence and metastasis of hepatocellular carcinoma. PMID: 20506647
A DEHP treatment-induced phenotype was identified with lack of glycogen accumula-tion and intracytoplasmic localization of beta-catenin which was associated with increased AFP gene expression. PMID: 20307648
The deficits observed in parental behavior of AFP knockout females could not be explained by any changes in olfactory function, novelty recognition or anxiety PMID: 20109458
Foxa1 acts as a pioneer transcription factor in de novo activation of Afp, by exploiting a lack of methylation at juxtaposed transposed elements PMID: 20348100
The level of AFP mRNA in peripheral blood may indicate recurrence and/or metastasis after curative resection of hepatocellular carcinoma. PMID: 12113675
AFP affects cell proliferation by binding its receptor to trigger the signal transduction pathway of cAMP-PKA and alter the expression of K- ras p21 genes PMID: 12118941
A murine Nkx2.8 was isolated from the Hepal-6 cell line and showed oligonucleotide binding competitive with fetoprotein transcription factor. PMID: 12167706
The mouse alpha-fetoprotein promoter is repressed in HepG2 hepatoma cells by hepatocyte nuclear factor-3 PMID: 12215259
AFP has a role in female fertility, but not in male fertility or survival, in mice PMID: 12297623
mouse and human alpha-fetoprotein enhancers are strikingly different PMID: 15028291
AFP protects the developing female brain from the adverse effects of prenatal estrogen exposure and clarify a long-running debate on the role of this fetal protein in brain sexual differentiation PMID: 16479017
30-kDa protein is a novel isoform of AUF1 family and is the main component of the DAP-II complex that binds to the DAS sequence PMID: 16514630
The sex difference in the ability to show preovulatory LH surges depends on the prenatal actions of estrogens in the male hypothalamus, is lost in Afp(-/-) females. PMID: 18202134
data indicate that this enhancer region is required for alpha-fetoprotein and albumin activation early in liver development and alpha-fetoprotein reactivation during liver regeneration PMID: 19782060

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.