Recombinant Mouse BDNF Protein

Name: Recombinant Mouse BDNF Protein
Brand: Beta LifeScience
SKU: BL-2578PS
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Mouse
Synonym	GP145-TrkB/GP95-TrkB,Trk-B, Neurotrophic tyrosine kinase receptor type 2, TrkB, tyrosine kinase, Ntrk2,Trkb, BDNF/NT-3 growth factors receptor.
Background	BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. BDNF is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the cns. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (ltp), long-term depression (ltd), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Description	BDNF expressed in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 409a.a. (32-429a.a.) and having a molecular weight of 45.7kDa (Molecular size on SDS-PAGE will appear at approximately 57-70kDa under reducing conditions).BDNF is expressed with an 11a.a. His tag at C-Terminus and purified by unique purification methods.
Source	Sf9
AA Sequence	CPTSCKCSSARIWCTEPSPG IVAFPRLEPN SVDPENITEI LIANQKRLEI INEDDVEAYV GLRNLTIVDS GLKFVAYKAFLKNSNLRHIN FTRNKLTSLS RRHFRHLDLS DLILTGNPFT CSCDIMWLKT LQETKSSPDT QDLYCLNESSKNMPLANLQI PNCGLPSARL AAPNLTVEEG KSVTLSCSVG GDPLPTLYWD VGNLVSKHMN ETSHTQGSLRITNISSDDSG KQISCVAENL VGEDQDSVNL TVHFAPTITF LESPTSDHHW CIPFTVRGNP KPALQWFYNGAILNESKYIC TKIHVTNHTE YHGCLQLDNP THMNNGDYTL MAKNEYGKDE RQISAHFMGR PGVDYETNPNYPEVLYEDWT TPTDIGDTTN KSNEIPSTDV ADQSNREHAA ALEHHHHHH.
Purity	Greaterthan 95.0% as determined by SDS-PAGE.
Endotoxin	<1.0 EU per μg by the LAL method.
Formulation	BDNF protein solution (0.5mg/ml) contains PhosphateBuffered Saline (pH 7.4) and 10% glycerol.
Stability	Recombinant protein is stable for 12 months at -70°C
Usage	For Research Use Only
Storage	Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.