Recombinant Mouse BLMH Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0417
Recombinant Mouse BLMH Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0417
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Mouse |
Accession | NP_848760.1 |
Synonym | AI035728, Bh, Bmh |
Background | The papain superfamily member bleomycin hydrolase (BLMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. The only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The papain superfamily member bleomycin hydrolase (BLMH) is a neutral cysteine protease with structural similarity to a 2S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent. BLMH is an essential protectant against BLM-induced death and has an important role in neonatal survival and in maintaining epidermal integrity. Sequencing revealed several putative sites phosphorylated by different types of protein kinases, but no signal sequence, transmembrane domain, N-linked glycosylation site or DNA-binding motif. |
Description | A DNA sequence encoding the mouse BLMH (NP_848760.1) (Asn 2-Glu 455) was expressed, with a His tag at the N-terminus. |
Source | E.coli |
Predicted N Terminal | Met |
AA Sequence | Asn 2-Glu 455 |
Molecular Weight | The recombinant mouse BLMH consisting of 461 a.a. and has a calculated molecular mass of 53.3 kDa. rmBLMH migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions. |
Purity | >95% as determined by SDS-PAGE |
Endotoxin | Please contact us for more information. |
Bioactivity | Measured by its ability to hydrolyze Met-AMC. The specific activity is >500 pmoles/min/ug. |
Formulation | Lyophilized from sterile 50mM Tris, 0.15M NaCl, 10% glycerol, pH 8.0. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Target Details
Target Function | The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B-aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. |
Subcellular Location | Cytoplasm. Cytoplasmic granule. |
Protein Families | Peptidase C1 family |
Database References |
Gene Functions References
- Blmh interacts with diverse cellular processes--lipoprotein, amino acid and protein, carbohydrate, and energy metabolisms, detoxification, antioxidant defenses--that are essential for normal kidney homeostasis. PMID: 24913063
- Bleomycin hydrolase protects against neurodegeneration associated with elevated homocysteine thiolactone levels in hyperhomocysteinemia and Alzheimer's disease. PMID: 22227865
- bleomycin hydrolase functions as an MHC class I epitope-processing protease PMID: 12140188
- BH does not play a major role either in generating or destroying class I major histocompatibility antigen (MHC)-presented peptides that bind to the MHC in living cells. These results point to redundant functions between peptidases. PMID: 17513741