Recombinant Mouse CADM1 Protein (C-6His)

Name: Recombinant Mouse CADM1 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-2245NP
Availability: InStock

BL-2245NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Description	Recombinant Mouse Cell Adhesion Molecule 1 is produced by our Mammalian expression system and the target gene encoding Gln48-His388 is expressed with a 6His tag at the C-terminus.
Accession	Q8R5M8
Synonym	Cell adhesion molecule 1;Immunoglobulin superfamily member 4;IgSF4;Nectin-like protein 2;NECL-2;Synaptic cell adhesion molecule;SynCAM;Tumor suppressor in lung cancer 1;TSLC-1
Gene Background	Cell adhesion molecule 1(CADM1) is a single-pass type I membrane protein and belongs to the nectin family. It contains 2 Ig-like C2-type (immunoglobulin-like) domains and 1 Ig-like V-type (immunoglobulin-like) domain. CADM1 acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. CADM1 may contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, it may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. The protein acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly. It may be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. CADM1 may play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.
Molecular Mass	39.2 KDa
Apmol Mass	60-100 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and NECTIN3 in a Ca(2+)-independent manner. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ T-cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. By interacting with CRTAM and thus promoting the adhesion between CD8+ T-cells and CD8+ dendritic cells, regulates the retention of activated CD8+ T-cell within the draining lymph node. Required for the intestinal retention of intraepithelial CD4+ CD8+ T-cells and, to a lesser extent, intraepithelial and lamina propria CD8+ T-cells and CD4+ T-cells. Interaction with CRTAM promotes the adhesion to gut-associated CD103+ dendritic cells, which may facilitate the expression of gut-homing and adhesion molecules on T-cells and the conversion of CD4+ T-cells into CD4+ CD8+ T-cells. Acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly. May be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. May play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.
Subcellular Location	Cell membrane; Single-pass type I membrane protein. Cell junction, synapse, synaptic cell membrane; Single-pass type I membrane protein.
Protein Families	Nectin family
Database References	KEGG: mmu:54725 STRING: 10090.ENSMUSP00000083073 UniGene: Mm.234832
Tissue Specificity	Expressed dominantly in epithelial cells but not expressed in fibroblast cells (at protein level). Expressed in the T-cell area of lymph nodes, specifically in CD8+ and CD4- CD8- dendritic cells (at protein level). Expressed in CD8+ dendritic cells in the

Gene Functions References

alternative splicing can give rise to both shedding-susceptible and shedding-resistant CADM1 (cell adhesion molecule 1) variant proteins. PMID: 28393893
SynCAM1, Neuroligin-1B and Neuroligin-2A were overexpressed in newborn neurons in the dentate gyrus of 7- to 9-week-old mice. SynCAM1 increased the morphological maturation of dendritic spines and mossy fiber terminals while Neuroligin-1B increased spine density. In contrast, Neuroligin-2A increased both spine density and size as well as GABAergic innervation and resulted in a drastic increase of neuronal survival. PMID: 27473321
The results of this study demonstrate that the synapse-organizing adhesion molecule SynCAM 1 has pronounced effects on neuronal connectivity in the hippocampal CA3 area. PMID: 27413156
Cadm1 is a direct target of NFATc1, which is induced by RANKL through epigenetic modification, and regulates osteoclastic bone resorption in a negative feedback manner. PMID: 28414795
Data show that cell adhesion molecule 1 (SynCAM 1) generally prolongs the lifetime of spines. PMID: 27053173
This experiments show that the survival rate of adult-generated neurons is increased by overexpressing SynCAM 1 PMID: 26332750
Our study reveals a novel mechanism of chronic NF-kappaB activation by CADM1 in HTLV-1 infected cells PMID: 25774694
Study demonstrates that polysialic acid-SynCAM 1 defines a subpopulation of NG2 cells and identifies the presence of polysialic acid-NRP2 in microglia PMID: 25752299
CADM1 appeared to sustain gap junctional intercellular communication among alpha-cells via homophilic binding and, based on this action, CADM1 appears to prevent excessive secretion of glucagon from alpha-cells. PMID: 22513384
This study demonistrated that SynCAM 1 in the synaptic organization of the rod visual pathway and provide evidence for novel roles of synaptic adhesion in the structural and functional integrity of ribbon synapses. PMID: 23982969
Necl-2 co-localizes with ErbB4 in hippocampus and and regulates ErbB4 signaling in cultured neurons. PMID: 23769722
Loss of SynCAM1 modulates cocaine effects on spine structures in the nucleus accumbens. PMID: 23169347
The bone volume fraction did not differ between the CADM1 knockout mice and the wild type mice for both 8-week old and 11-week old mice. PMID: 23541110
Cadm1 is indeed a bona fide tumor suppressor gene and provide new insights into genetic partners that co-operate in tumorigenesis when Cadm1-expression is lost. PMID: 22553910
These results indicated that CADM1 was a novel osteoblastic adhesion molecule that is expressed transiently during osteoblastic maturation, and a useful diagnostic marker for osteosarcoma cells. PMID: 23142238
Cadm1 specifically interacts with Mupp1, and may form a ternary complex with Mupp1-GABBR2 in the cerebellum. PMID: 22994563
Epigenetic silencing complexity in the promoter region of Cadm1 is not only defined by DNA hypermethylation, but high nucleosome occupancy, altered nucleosome positioning and 'bivalent' histone modifications. PMID: 22701659
disruption of SynCAM1-dependent astroglial function results in behavioral abnormalities similar to those described in animals model of attention-deficit hyperactive disorder PMID: 22558465
Cadm1 expression in the hair follicle plays a role in autoimmune alopecia. PMID: 22210910
Results suggest that although the Foxp2 transcription factor does not target Cadm1, Cadm1 at the synapses of Purkinje cells and parallel fibers is necessary for ultrasonic vocalization (USV) function. PMID: 22272290
These results clarify the detailed mechanism of membrane-proximal cleavage of CADM1, suggesting the possibility of RIP-mediated CADM1 signaling. PMID: 22172944
Synaptic cell adhesion molecule SynCAM 1 self-assembles laterally via its extracellular, membrane-proximal immunoglobulin domains 2 and 3, generating SynCAM oligomers and instructing interactions of SynCAM 1 across the nascent and mature synaptic cleft. PMID: 21926970
IGSF4 plays a central role in T cell functioning by dual independent mechanisms, control of T cell receptor signaling and control of T cell-antigen-presenting cell interaction. PMID: 22084409
SynCAM1 mediates astrocyte-to-astrocyte and astrocyte-to-GnRH neuron adhesiveness in the hypothalamus. PMID: 21486931
SynCAM1 is expressed in astrocytes and contributes to erbB4 receptor-mediated control of female sexual development. PMID: 21486934
From pachytene spermatocytes at stage VI to step 4 spermatids, RA175 was not detected by the enzyme-labelled antibody method and was faintly detected by the indirect immunofluorescence method. PMID: 21486398
results indicate that CADM1d is a specific neuronal isoform that enhances nerve-mast cell interaction, and they suggest that nerve-mast cell interaction may be reinforced as the brain grows mature because CADM1d becomes predominant PMID: 21482734
The mutated CADM1 showed morphological abnormalities, including shorter dendrites, and impaired synaptogenesis in neurons. PMID: 21364653
Overexpression of SynCAM 1 in transgenic mice promotes excitatory synapse number, while loss of SynCAM 1 results in fewer excitatory synapses. SynCAM 1 also functions at mature synapses to alter their plasticity by regulating long-term depression. PMID: 21145003
this study demonstrates that CADM1 plays roles in regulating emotional behaviors, social behaviors and motor performances, and that CADM1 has important implications for psychiatric disorders with disruptions in social behavior, such as autism. PMID: 20450890
Analysis of serial sections of perinatal Ncam(-/-) brain revealed that polySia-SynCAM 1 is expressed exclusively by NG2 cells, a multifunctional glia population that can receive glutamatergic input via unique neuron-NG2 cell synapses. PMID: 20479255
SynCAM 1 acts in developing neurons to shape migrating growth cones and contributes to the adhesive differentiation of their axo-dendritic contacts PMID: 20368431
Cell adhesion molecules between spermatogenic and Sertoli cells play essential roles in spermatogenesis. PMID: 19337787
The Tslc1 gene is mapped on mouse chromosome 9, a synteny of human chromosome 11q, and is composed of ten exons, the exon-intron junctions being highly conserved between human and mouse. PMID: 12242005
results showing that SgIGSF mediates the adhesion of mast cells to fibroblasts and that the transcription of SgIGSF is critically regulated by MITF. PMID: 12456501
SgIGSF on the surface of spermatogenic cells binds to some membrane molecules on Sertoli cells in a heterophilic manner and thereby may play diverse roles in the spermatogenesis PMID: 12606335
Possibly involved in localization of a transmembrane protein(s) through Pals2. PMID: 12826663
SgIGSF has a role in survival of cultured mast cells PMID: 15158462
SgIGSF and KIT independently play significant roles in cell migration PMID: 15474495
the tscl1 protein was localized in the brain neuroepithelium at embryonic day 9.5, neural crest at E10.5, motor neurons at E10.5, and olfactory epithelium at E16.5. PMID: 15707673
The spatiotemporal patterns of the expression suggest that IGSF4 plays crucial roles in the development of both telencephalon and cerebellum. PMID: 15862624
RA175-based cell junction is necessary for retaining elongating spermatids in the invagination of Sertoli cells for their maturation and translocation to the adluminal surface for timely release. PMID: 16382161
Results suggest that MITF and SgIGSF appear essential for the development and survival of mast cells in tissues of adult WBB6F1-KitW/KitW-v mice. PMID: 16605125
Tslc1 is essential for normal spermatogenesis in mice. PMID: 16611999
TSLC1/IGSF4 expression is indispensable for the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. PMID: 16612000
results establish an essential role for Tslc1 in spermatozoal maturation & motility, distinct from other members of the nectin family PMID: 16837733
These results indicate that TSLC1 is a novel interameloblast adhesion molecule that may be downregulated during ameloblastic tumorigenesis. PMID: 17300670
We propose that SgIGSF is a novel and functional biliary epithelial cell adhesion molecule that is expressed for a limited time during active bile duct/ductule formation. PMID: 17326163
The retrograde response to axotomy of spinal motoneurons involves a rapid down-regulation of SynCAM1 mRNA, indicating that down-regulation of synaptic adhesion molecules plays a role in postinjury synapse elimination PMID: 17492651
In the Ra175(-/-) elongating spermatid, Par-3 was absent, and Jam-C was absent or abnormally localized. PMID: 18055550

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

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