Recombinant Mouse Mer Protein (Fc Tag)

Name: Recombinant Mouse Mer Protein (Fc Tag)
Brand: Beta LifeScience
SKU: BLPSN-3337
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	Fc
Host Species	Mouse
Accession	Q60805
Synonym	Eyk, Mer, nmf12, Nyk
Background	Proto-oncogene tyrosine-protein kinase MER (MERTK) is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. MERTK is localized in membrane and is no expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. This protein is highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver. MERTK regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. MERTK plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. Defects in MERTK are the cause of retinitis pigmentosa type 38.
Description	A DNA sequence encoding the mouse MERTK (Q60805) extracellular domain (Met 1-Phe 498) was was fused with the Fc region of human IgG1 at the C-terminus.
Source	HEK293
Predicted N Terminal	Gly 19
AA Sequence	Met 1-Phe 498
Molecular Weight	The secreted recombinant mouse MERTK/Fc chimera is a disulfide-linked homodimer. The reduced monomer consists of 721 a.a. and has a predicted molecular mass of 79.3 kDa. As a result of glycosylation, rm MERTK/Fc monomer migrates as an approximately 130 kDa band in SDS-PAGE under reducing conditions.
Purity	>90% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
Subcellular Location	Membrane; Single-pass type I membrane protein.
Protein Families	Protein kinase superfamily, Tyr protein kinase family, AXL/UFO subfamily
Database References	KEGG: mmu:17289 STRING: 10090.ENSMUSP00000014505 UniGene: Mm.239655
Tissue Specificity	Expressed predominantly in the hematopoietic lineages: macrophages, NK cells, NKT cells, dendritic cells and platelets.

Gene Functions References

These results suggest that MerTK inhibition impaired phagocytic function of the retina PMID: 29310530
MerTK does not play an essential role in the phagocytosis of S. aureus but attenuates inflammation induced by staphylococcal LTA through blocking NF-kappaB activation. PMID: 28528507
Tumor macrophage expression of Mertk is a therapeutic target to prevent tumor recurrence following radiation therapy. PMID: 27602953
Hypercapnic Acidosis Regulates Mer Tyrosine Kinase Receptor Shedding and Activity. PMID: 29286859
GC B cell-intrinsic sensing of self-RNA, but not self-DNA, from dead cells in GCs drives enhanced GC responses in Mer(-/-) mice. Mer loss in dendritic cells promotes enhanced T cell activation and proinflammatory cytokine production. Mer immunoregulatory signaling in APCs regulates B cell selection and autoimmunity. The phagocytic and immunomodulatory functions of Mer regulate GC responses preventing autoimmunity. PMID: 29118245
this study shows that viral infection sensitizes fetal membranes by MERTK Inhibition PMID: 28916522
Monocyte-induced MerTK cleavage on proreparative MHCII(LO) cardiac macrophages is a novel contributor to myocardial ischemic reperfusion injury. PMID: 28851810
evidence that proteolytic cleavage of the macrophage efferocytosis receptor c-Mer tyrosine kinase (MerTK) reduces efferocytosis and promotes plaque necrosis and defective resolution. PMID: 28067670
these results suggest, for the first time, that MerTK is an intracellular negative feedback regulator that inhibits the inflammatory response of lipoteichoic acid -stimulated macrophages PMID: 27419619
the reciprocal activation of Axl and Mer receptor tyrosine kinases has a major impact on the outcome of renal inflammation. PMID: 27527599
Reduced transcription of Mertk, rather than differences in MERTK protein structure, determines the reduced efficiency of apoptotic cell clearance in the Aath4a(DBA/DBA) mice, which, in turn, contributes to their increased susceptibility to atherosclerosis. PMID: 28473436
Axl, Mertk and Tyro3 receptors are not required for Zika virus entry and infection. PMID: 28423319
Signaling through the Mer proto-oncogene tyrosine kinase (MerTK) receptor in cultured macrophages and in sterile inflammation in vivo promotes specialized proresolving mediator (SPM) biosynthesis by a mechanism involving an increase in the cytoplasmic:nuclear ratio of a key SPM biosynthetic enzyme, 5-lipoxygenase. PMID: 27199481
These studies define the clearance of infected, apoptotic neutrophils by dendritic cells and Mer receptor signaling as central to the early immune evasion strategies of L. major. PMID: 26658192
Tyro3 gene dosage modulates Mertk-associated retinal degeneration, provide strong evidence for a direct role for TYRO3 in RPE phagocytosis, and suggest that an eQTL can modify a recessive Inherited photoreceptor degenerations. PMID: 26656104
Activation of Mertk synergized with interferon-beta to tighten cell junctions and prevent virus transit across brain microvascular endothelial cells. PMID: 26523970
results establish TAM receptors as both controllers of microglial physiology and potential targets for therapeutic intervention in central nervous system disease PMID: 27049947
Loss of Mertk alters expression of micrornas in retinal pigment epithelial cells. PMID: 25604732
These results suggest that TAM receptors support NSCs survival, proliferation and differentiation by regulating expression of neurotrophins, especially the nerve growth factor. PMID: 25514676
Mertk deficiency affects not only phagocytosis but also cell shape and migration PMID: 25617898
The results indicate that Axl and Mer receptors cooperatively regulate the systemic immune tolerance to male germ cell antigens. PMID: 25403570
Enhanced Mer signaling during the recovery phase increases the abundance and activity of LXR to inactivate the inflammatory response in macrophages PMID: 25714463
nuclear receptor agonists increase MerTK and Axl expression on plaque-associated immune cells, consequently licensing their phagocytic activity and promoting plaque clearance. PMID: 25904803
Mertk expression is required for optimal B-cell antigen presentation, which is, in turn, required in this model for optimal T cell activation and subsequent T cell-dependent B cell differentiation. PMID: 24768065
adiponectin elicited Mer expression and Mer-dependent efferocytosis in macrophages similar to cells stimulated with C1q. PMID: 24942043
Optimal TAM signaling requires coincident TAM ligand engagement of both its receptor and the phospholipid phosphatidylserine regulating TAM receptor tyrosine kinases Tyro3, Axl, and Mer and their ligands Gas6 and Protein S. PMID: 25265470
data suggest that MerTK cleavage contributes to the acute regulation of RPE phagocytosis by limiting POS binding to the cell surface. PMID: 25538233
Inhibition of the Gas6 receptor Mer or therapeutic targeting of Gas6 by warfarin reduced myeloma burden and improved survival in a systemic model of myeloma. PMID: 25102945
Mer mediates quiescence and chemotherapy resistance in a CNS co-culture model and causes CNS infiltration in immunodeficient mice. PMID: 25428221
Data indicate that TAM receptor tyrosine kinases Axl and Mer had distinct roles as phagocytic receptors. PMID: 25194421
Data indicate that MerTK, a receptor kinase, was essential for the engulfment of pyrenocytes by the central macrophages at erythroblastic islands. PMID: 24659633
Data collectively and directly link efferocytosis to wound healing in the heart and identify Mertk as a significant link between acute inflammation resolution and organ function. PMID: 23836795
the engulfment of apoptotic cells by resident peritoneal macrophages proceeds in two steps: binding to Tim4, a PtdSer receptor, followed by MerTK-mediated cell engulfment PMID: 24515440
Axl and Mer (TAM) receptor tyrosine kinases (RTKs) developed persistent inflammatory liver damage resembling AIH. Tyro3(-/-)Axl(-/-)Mer(-/-) triple mutant (TAM(-/-)) mice exhibited chronic hepatitis PMID: 23799121
Adult brain neurogenesis is reduced in the hippocampus of the Tyro3-/-Axl-/-Mertk-/- triple-knockout & Axl-/-Mertk-/- double-knockout mouse brains, but not in single Mertk-/- knockouts. PMID: 24244024
Chronic systemic inflammation and autoimmune disorders in the Tyro3, Axl and Mertk knockout mice cause neuronal damage and death. PMID: 23840307
studies reveal a novel role for astrocytes in mediating synapse elimination in the developing and adult brain, identify MEGF10 and MERTK as critical proteins in the synapse remodelling underlying neural circuit refinement PMID: 24270812
Data indicate the two phagocytic proteins, Mer receptor tyrosine kinase (MerTK) and Milk fat globule EGF-like factor 8 (MFG-E8), were transiently up-regulated by macrophages/microglia after focal brain ischemia in vivo. PMID: 24101459
Data indicate that azoxymethane (AOM) and dextran sulfate sodium (DSS)-induced inflammation-associated cancer is exacerbated in mice lacking Axl and Mer receptor tyrosine kinases. PMID: 23878224
MerTK signaling may suppress antitumor immunity through suppression of CD8+ T lymphocyte proliferation. PMID: 23867499
Mer deficiency in knockout mice results in long-term accumulation of apoptotic cells primarily in germinal centers and not in the T cell zone, marginal zone, or red pulp areas of the spleen. PMID: 23319738
Results demonstrate that Protein S and Gas6 function as independent, bona fide Mer ligands, and are, to a first approximation, interchangeable with respect to Mer-driven phagocytosis in the retina. PMID: 23259948
Mer of the TAM-family receptors is responsible for mediating transcriptional HGF production through a RhoA-dependent pathway. PMID: 22740630
These results suggest that tubby is a ligand to facilitate microglial phagocytosis through MerTK for the maintenance of CNS homeostasis. PMID: 22884297
Inhibiting Mer receptor tyrosine kinase suppresses STAT1, SOCS1/3, and NF-kappaB activation and enhances inflammatory responses in lipopolysaccharide-induced acute lung injury. PMID: 22427680
Novel insight into the mechanism of TAM RTKs (Tyro3, Axl, Mer receptor tyrosine kinases)in regulating male fertility. PMID: 19602523
A TRIF-mediated pattern recognition receptor signaling cascade requires NADPH oxidase to activate PKCdelta and then p38, culminating in ADAM17-mediated proteolysis of MerTK. PMID: 21828049
TNF family members play a role in protecting photoreceptors of Mertk(nmf12) homozygotes from cell death. PMID: 21436282
ERG from mer(kd) mice of different ages showed inner retinal dysfunction in mer(kd) mice. This and faster degeneration in mer(kd) mice may produce retinal environment unresponsive to neuroprotection from subretinal electrical stimulation. PMID: 21467171
disrupted expression leads to enhanced marginal zone B-cell responses PMID: 20822883

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