Recombinant Mouse SCF Protein (His Tag)

Name: Recombinant Mouse SCF Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-4157
Availability: InStock

Collections: Cytokines and growth factors, Growth factors and receptors, Recombinant proteins, Recombinant stem cell factor (scf) proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Tag	His
Host Species	Mouse
Accession	P20826
Background	Similar to Kit ligand precursor (C-kit ligand) , also known as Stem cell factor (SCF), Mast cell growth factor (MGF) or Hematopoietic growth factor KL. SCF/C-kit ligand is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. SCF/C-kit ligand stimulates the proliferation of mast cells. This protein is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It may act synergistically with other cytokines, probably interleukins SCF/C-kit ligand is the ligand for the tyrosine kinase receptor c-kit, which is expressed on both primitive and mature hematopoietic progenitor cells. In vitro, SCF/C-kit ligand synergizes with other growth factors, such as granulocyte colony-stimulating factor (G-CSF), granulocyte macrophage- colony- stimulating factor, and interleukin-3 to stimulate the proliferation and differentiation of cells of the lymphoid, myeloid, erythroid, and megakaryocytic lineages. In vivo, SCF/C-kit also synergizes with other growth factors and has been shown to enhance the mobilization of peripheral blood progenitor cells in combination with G-CSF. In phase I/II clinical studies administration of the combination of SCF and G-CSF resulted in a two- to threefold increase in cells that express the CD34 antigen compared with G-CSF alone.
Description	A DNA sequence encoding the amino acids (Met 1-Ala 189) of mouse KITL (P20826-1) was fused with a His tag at the C-terminus.
Source	Baculovirus-Insect Cells
Predicted N Terminal	Lys 26
AA Sequence	Met 1-Ala 189
Molecular Weight	The secreted recombinant mouse KITL consists of 175 a.a. and has a calculated molecular mass of 19.7 kDa. The recombinant protein migrates as an approximately 23 kDa band in SDS-PAGE under reducing conditions.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	1. Measured by its binding ability in a functional ELISA. Immobilized mouse KITL at 2 ug/ml (100 ul/well) can bind mouse CD117 with a linear ranger of 1.28-32 ng/ml.2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells (Kitamura,T. et al. 1989, J. Cell Physiol. 140:323.). The EC50 for this effect is typically 0.5-5 ng/ml.3. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 5-30 ng/mL.
Formulation	Lyophilized from sterile 20mM Tris, 50mM NaCl, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
Subcellular Location	[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Single-pass type I membrane protein. Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, filopodium.; [Soluble KIT ligand]: Secreted.
Protein Families	SCF family
Database References	KEGG: mmu:17311 STRING: 10090.ENSMUSP00000100920 UniGene: Mm.45124
Tissue Specificity	Expressed in the cochlea.

Gene Functions References

These findings support the view that SCF, in addition to its pro-proliferative effects, is important for the differentiation of MCs. PMID: 27619074
In vitro, SCF induced the phosphorylation of p38 MAPK and cofilin, leading to the migration of cardiac stem cells. PMID: 28986094
MAPK3/1 participates in primordial follicle activation through mTORC1-KITL signaling. PMID: 28218391
MicroRNA-205 maintains T cell development following stress by regulating Foxn1 and its two regulated targets, stem cell factor and ccl25, following stress. PMID: 27646003
Bone marrow adipocytes synthesize SCF, promoting hematopoietic stem cell proliferation/regeneration. PMID: 28714970
SCF/c-kit signaling may potentiate chronic hypoxia-induced vascular remodeling by modulating ERK activation. Inhibition of c-kit activity may be a potential strategy to alleviate PH PMID: 26705118
Kit ligand regulates the subcellular localization of FOXO3 in the neonatal mouse ovary. PMID: 26507072
The results of this study indicate that c-kit/KITL signalling also occurs in the human ovary, as established in various animal models, and may involve previously unknown autocrine signalling PMID: 26008799
Streptozotocin-induced diabetes was induced in cKit-mutant mice with low cKit expression in their endothelial cells. PMID: 26978025
study suggests extracellular HMGB1 participates in NLRP3 inflammasome activity and regulates IL-1beta associated sterile inflammation induced by MWCNT PMID: 25779020
These results show that mast cell deficiency, in the absence of Kit mutations, plays no role in the regulation of weight gain or insulin resistance. PMID: 25955205
Stem cell factor is essential for preserving reconstitution capacity of ex vivo expanded cord blood CD34(+) cells in Immunocompromised mice. PMID: 25899394
High stem cell factor expression is associated with breast cancer metastasis. PMID: 23577751
Cardiomyocyte-specific overexpression of hSCF promotes epicardial activation and myocardial arteriogenesis post MI. PMID: 25107671
SCF+G-CSF treatment in chronic stroke remodels neural circuits in the aged brain PMID: 23750212
Data reveal that SCF disrupts the endothelial adherens junction and enhances vascular leakage, and suggest that anti-SCF/cKit therapy may hold promise as a potential therapy for the treatment of hyperpermeable vascular diseases. PMID: 24790137
results show that the c-kit/mSCF/MMP-9 axis regulates IL-23 gene expression in DCs to control IL-17 production in the lung. PMID: 24829419
Data indicate that insulin-like growth factor-1 (IGF1) stimulated s tem cell factor (Kitl/KITLG) protein and mRNA expression and promoter activity by activating several signaling pathways. PMID: 24116170
Wnt7a triggers melanocyte stem cell differentiation through beta-catenin activation, and Kitl might induce following migration of melanoblasts to epidermis PMID: 23702581
data and the identification of c-kit/Kit-ligand clusters at cell contacts suggest that membrane-bound Kit ligand captures cell surface-expressed c-kit PMID: 22637532
A major role is described for SCF in the homeostatic control of mast cell activation with potential relevance to mast cell-driven disease and the development of novel approaches for the treatment of allergic disorders. PMID: 22529299
Proliferation and the basal/luminal cell composition of cells isolated from the proximal region of prostatic ducts (the stem cell niche) is regulated in part by opposing effects of stem cell factor and endogenous TGF-beta. PMID: 22024978
Data show tumor-promoting potential of mast cells could be augmented by molecules released from damaged tumor cells through cooperative stimulation of stem cell factor (SCF) and ligand for Toll-like receptor 4 (TLR4). PMID: 21877248
membrane-bound Steel factor controls germ cell motility within a "motility niche" that moves through the embryo with the germ cells PMID: 21998739
These experiments revealed a new function of stem cell factor in chemokine receptor coupling. PMID: 20427772
The altered sorting of KitL is dispensable for hematopoietic and melanogenic lineages, yet is crucial in the testicular environment, where the basal membranes of adjacent polarized Sertoli cells form a niche for the proliferating spermatogonia. PMID: 19874813
Results suggest that the c2j tyrosinase defect can be rescued in part by stem cell factor in the ears and tail. PMID: 19682281
diabetic mice show a decline in the number of interstitial cells of Cajal and impairment in the ultrastructures, and these abnormalities are attributed to a deficiency in the endogenous SCF but are not related to hyperglycemia PMID: 19875700
cell surface form of SCF mainly promotes the proliferation of hemopoietic stem cells PMID: 11811778
data show that Kitl induces telomerase activity in mitotic spermatogonia and increases the mRNA levels of both the catalytic subunit form and the telomerase RNA template PMID: 11950883
intracellular signaling pathway of SCF/KIT-mediated cell migration PMID: 11964302
role of fibronectin and stem cell factor in melanocyte growth, differentiation and migration PMID: 12028583
involvement of Kit/SCF at different stages of spermatogenesis PMID: 12080001
involved in the induction of LTP and the blockade of LTP by rmSCF might be due to an occlusion of SCF/c-kit signaling PMID: 12137920
different isoforms of SCF may use different biochemical pathways in stimulation of survival and/or proliferation of erythroid cells PMID: 12149209
Stem cell factor radioprotection is due to PLC gamma 1-dependent negative regulation of ionizing radiation induced neutral sphingomyelinase stimulation PMID: 12149210
stem cell factor (kit ligand) stimulated neurogenesis in vitro and in vivo PMID: 12163450
identification of an allelic series of mutations in the kit ligand gene PMID: 12242244
effects of ethylnitrosourea-induced Kitl point mutations on survival and peripheral blood cells of Kitl(Steel) mice PMID: 12242245
SCF is a potent eosinophil degranulator and activator that may play a number of roles during an inflammatory/immune response. PMID: 12453875
Levels of E2A protein expression in B cell precursors are stage-dependent and inhibited by stem cell factor, mainly from posttranscriptional regulation. PMID: 12482503
data suggest that FoxO3 plays an important role in kit ligand-mediated survival of hematopoietic progenitors PMID: 12691919
Phosphatidylinositol 3-kinase is essential for kit ligand-mediated survival. PMID: 12960281
Stem cell plays a significant role in restoring hepatocyte proliferation in IL-6 knockout mice following hepatectomy. PMID: 14597766
that although transmembrane-SCF does not appear to play a role in the homing of transplanted cells to the bone marrow, it is critical in the lodgment and detainment of HSC within their hemopoietic "niche." PMID: 14662336
SLF and SDF-1alpha have a synergistic effect in fetal hematopoietic stem cells PMID: 15024423
XPA (-/-), SCF-Tg mice did not develop skin cancers after repeated exposures to UVB for 30 wk at a total dose of 72 J per cm(2) PMID: 15191564
C-terminal valine defines a specific endoplasmic reticulum export signal in Kitl PMID: 15475566
Cessation of SCF treatment results in the rapid reduction of cutaneous skin mast cell populations by apoptosis. PMID: 15502858
role for SCF in cell migration and survival in the developing cortex; SCF is a survival factor, but not a mitogen or a differentiation factor for neural stem cells PMID: 15530856

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.