Recombinant Rhesus CD16 Protein (His & AVI Tag), Biotinylated
Beta LifeScience
SKU/CAT #: BLPSN-0710
Recombinant Rhesus CD16 Protein (His & AVI Tag), Biotinylated
Beta LifeScience
SKU/CAT #: BLPSN-0710
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His&AVI |
Host Species | Rhesus |
Accession | NP_001258584.1 |
Background | Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes. |
Description | A DNA sequence encoding the rhesus CD16 (NP_001258584.1) extracellular domain (Met 1- Gln 208) was fused with a c-terminal His tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
Source | HEK293 |
Predicted N Terminal | Gly 17 |
AA Sequence | Met 1- Gln 208 |
Molecular Weight | The recombinant rhesus CD16 consists of 223 a.a. and has a calculated molecular mass of 25.3 kDa. |
Purity | >92% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | 1. Measured by its ability to bind human IgG1 in a functional ELISA.2. Labeling ratio of biotin to protein: 0.4 |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |