Recombinant Human Thrombopoietin Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4474
Recombinant Human Thrombopoietin Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-4474
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Synonym | MGDF, MKCSF, ML, MPLLG, THCYT1, TPO |
Background | Thrombopoietin (TPO or THPO), also known as myeloproliferative leukemia virus ligand (c-Mpl), is a hematopoietic growth factor belonging to the EPO/TPO family. The thrombopoietin protein is produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. Thrombopoietin protein stimulates both proliferation of progenitor megakaryocytes and their maturation to platelet-producing megakaryocytes, and also accelerates the recovery of platelets. Thrombopoietin protein is involved in cardiovascular disease as it regulates megakaryocyte development and enhances platelet adhesion/aggregation. It has been identified that surface c-MPL, the receptor for thrombopoietin protein, binds to the ligand and mediates the action. |
Description | A DNA sequence encoding the mature form of human THPO (NP_000451.1) (Ser22-Gly353) was fused with a His tag at the N-terminus. The purified protein was biotinylated in vitro. |
Source | HEK293 |
Predicted N Terminal | Ser 22 |
AA Sequence | Ser22-Gly353 |
Molecular Weight | The recombinant human THPO consists of 348 a.a. and predicts a molecular mass of 37.6 kDa. |
Purity | >90% as determined by SDS-PAGE. |
Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
Bioactivity | Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 10-60 ng/ml. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |